Abstract 3792: WISP-1 increases cell migration and ICAM-1 expression in human oral squamous cell carcinomas.

Abstract

Abstract Oral squamous cell carcinomas (OSCC) have a striking tendency to migration and metastasis to cervical lymph nodes. Wnt-1 induced secreted protein 1 (WISP-1) belongs to the CCN family (CTGF/CYR61/NOV), which is secreted cysteine-rich proteins. However, the effects of WISP-1 on migration and Intercellular adhesion molecule-1 (ICAM-1) expression in human OSCC are largely unknown. First, we found that the expression of WISP-1 in human OSCC tissues was higher than in normal oral tissues. We also demonstrated that WISP-1 enhanced cell migration and ICAM-1 up-regulation in human OSCC. Transfection of cells with ICAM-1 siRNA reduced WISP-1-mediated cell migration. WISP-1 activated αvβ3 integrin, ASK1, JNK/p38, c-Jun, and AP-1 signal transduction pathway, and WISP-1-induced expression of ICAM-1 and migration activity was inhibited by the αvβ3 antibody or inhibitor of ASK1, p38, JNK, and c-Jun. In addition, human OSCC were infected with lentivirus containing human WISP-1 shRNA which reduced the migratory ability and ICAM-1 expression. Over all, we characterized an important role for WISP-1 which regulates cell migratoin by increasing ICAM-1 expression in human OSCC via the αvβ3 integrin, ASK1, JNK/p38, c-Jun, and AP-1 signaling pathway. Citation Format: An-Chen Chang. WISP-1 increases cell migration and ICAM-1 expression in human oral squamous cell carcinomas. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 3792. doi:10.1158/1538-7445.AM2013-3792