Abstract 3785: Telomerase RNA component lncRNA regulates hsa-miR-320 family and promotes prostate cancer metastasis

Abstract

Abstract Telomerase RNA component (TERC) is a long non-coding (lncRNA) associated with prostate cancer progression and has novel prognostic biomarker potential. Differential methylation patterns of lncRNAs affect their expression levels, leading to cancer. This study aimed to profile and elucidate differential lncRNA expression patterns in prostate cancer (PCa). A 384-well plate of PCa associated lncRNA gene array panel was used in castration resistant PC-3 PCa cells vs androgen-sensitive PCa LNCaP cells. Annotation and enrichment analysis of lncRNA differential gene expression was performed using a human lncRNA sets database, LncSEA. Furthermore, digital droplet PCR (ddPCR) was used to verify the PCR array results. Up or down-regulation of ±2 and p <0.05 were considered significant. Decreased hsa-miR-320 gene family expression has been reported in various tumors including PCa. However, the precise mechanisms of hsa-miR-320 gene family in PCa remain to be elucidated. Thirty six of 84 lncRNAs were shown to be upregulated, while 14% of the lncRNA genes were downregulated. Notably, TERC lncRNA was upregulated in high metastatic PC-3 cells. Hypermethylation patterns of this lncRNA in high metastatic PC-3 cells was also shown by enrichment analysis. Furthermore, TERC lncRNA was demonstrated to regulate the hsa-miR-320 family, suggesting a correlation between TERC hypermethylation and downregulation of hsa-miR-320 gene family expression in PCa, as revealed by bioinformatics analysis. TERC lncRNA promotes PCa cell proliferation, migration, invasion and metastasis by sponging hsa-miR-320 family. This TERC/hsa-miR-320 interaction holds potential to understanding PCa prognostic mechanisms by lncRNAs and may also be targeted for novel therapeutics. Citation Format: Mandisa Mbeje, Jeyalakshmi Kandhavelu, Clement Penny, Zodwa Dlamini, Rahaba Marima. Telomerase RNA component lncRNA regulates hsa-miR-320 family and promotes prostate cancer metastasis. [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2023; Part 1 (Regular and Invited Abstracts); 2023 Apr 14-19; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2023;83(7_Suppl):Abstract nr 3785.