Abstract 3681: Facile fabrication of MRI-capable and NIR-resonant core-satellite nanomediators for photothermal therapy

Abstract

Abstract Although many techniques exist for fabricating near-infrared (NIR)-resonant and magnetic resonance imaging (MRI)-capable nanomediators for photothermal cancer therapy, preparing them in an efficient and scalable process remains a significant challenge. In this report, we develop two facile methods to fabricate both NIR-resonant and MRI-capable nanomediators utilizing well developed polysiloxane-containing polymer-coated iron oxide nanoparticles (IONPs). One of the methods is to attach multiple NIR-resonant nanoparticles onto a thiol-modified IONP core through one-step mixing with gold sulfide nanoparticles (Au2S NPs) to fabricate a core-satellite nanomediator. The other strategy is to covalently attach a NIR organic dye onto intact polysiloxane-containing polymer-coated IONPs through facile mixing with siloxane-modified IR820. The successful decoration with either Au2S NPs or IR820 is demonstrated for the resultant nanomediators by absorption spectra and transmission electron microscopy (TEM) images. It is estimated that 10 Au2S NPs and 2×104 dye molecules (loading capacity: 4.5 mg IR820/mg Fe) are attached onto the polymer-coated IONPs, respectively. The hydrodynamic sizes of these novel multifunctional nanomediators are ∼57.5 nm for IONP-Au2S NP and 28.2 nm for IONP-IR820, which are much smaller than traditional gold nanoshell-based designs. The photothermal efficiency of the IONP-Au2S NP and IONP-IR820 nanocomposite solutions under the irradiation of 885 nm NIR laser light is significantly enhanced compared to IONPs alone. The enhancement in photothermal efficiency by the newly developed nanomediators is also investigated in nude mice bearing SUM-159 tumor xenografts. The biodistribution data measured by inductively coupled plasma optical emission spectrometry (ICP-OES) reveal that ∼1% injection dose (ID) of IONP-Au2S NPs and ∼9% ID of IONP-IR820 accumulate into tumor tissue 24 h post intravenous injection at a dose of 20 mg Fe/Kg mouse body weight. The average tumor surface temperature recorded by an infrared camera is increased by 10.8 ± 1.2 °C for the mice administered with IONP-Au2S NPs and 25.7 ± 3.6 °C for the group injected with IONP-IR820, compared to 4.8 ± 0.5 °C for the PBS-injected control mice after laser irradiation with a power of 0.5 W for 10 min. Furthermore, our data reveal that the fabrication will not compromise the IONP core capability as an MRI contrast agent. Taken together, we demonstrate a facile technique to generate NIR-resonant and MRI-capable nanomediators from a polysiloxane-containing polymer-coated IONP platform. The as-developed dual functional nanomediators hold great promise for translatable MRI-guided photothermal cancer therapy. Citation Format: Hongwei Chen, Xiaoqing Ren, Hayley Paholak, Joseph Burnett, Feng Ni, Duxin Sun. Facile fabrication of MRI-capable and NIR-resonant core-satellite nanomediators for photothermal therapy. [abstract]. In: Proceedings of the 106th Annual Meeting of the American Association for Cancer Research; 2015 Apr 18-22; Philadelphia, PA. Philadelphia (PA): AACR; Cancer Res 2015;75(15 Suppl):Abstract nr 3681. doi:10.1158/1538-7445.AM2015-3681