Abstract
Introduction: Studies have demonstrated that Win55,212-2 induced pharmacological hypothermia and protected the neurological function after cardiopulmonary resuscitation (CPR) in a rat cardiac arrest (CA) model. However, the mechanism is still unclear. In this study, we hypothesized brain derived neurotrophic factor (BDNF) was involved in this mechanism. Methods: CA was induced by transoesophageal ventricular pacing in SD rats. 5min after onset of CA, CPR was started. After 30 min of ROSC, 30 animals were randomized into either Win55, 212-2 group (animals in drug groups were received continuous intravenous infusion of Win55,212-2 with 1 mg/kg/h for 4 h) or Normothermia group (animals were received 5% DMSO). Rats in normothermia group were maintained at 37°C with warming lamp though the whole experiment. Rats in drug group were warmed to 37°C increasingly after 4h of Win55,212-2 treatment. Brains of five animals in each group were harvested for detecting BDNF by RT-PCT and Westen blot at 24 h after ROSC respectively. Results: Temperatures of animals in Win55,212-2 group decreased to 34°C in 4 hours after ROSC, and the survival of which were longer than that of the control group. The expression of BDNF was significantly higher in the Win55, 212-2 group than that of control group. Conclusions: BDNF paticipated the neuroprotection of Win 55,212-2 after CPR in a rat CA model.
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