Abstract 3561: MicroRNA-214 inhibits prostate cancer cell proliferation, migration, invasion and increases drug sensitivity by targeting PTK6

Abstract

Abstract MicroRNAs (miRNAs) are involved in numerous physiological and pathological processes and play a critical role in cancer progression and the role miRNA-214 (miR-214) in prostate cancer remains elusive. In the present study, we investigated the effect of expression of miR-214 on cell survival/migration/invasion, cell cycle regulation, and apoptosis. Differential expression of miR-214 was found between Caucasian, African American prostate cancer cells compared with normal prostate epithelial cells. Expression of miR-214 in prostate cancer cells inhibited cell proliferation and colony forming capability, and induced apoptosis. miR-214 also inhibited cell migration and 3D spheroid invasion in prostate cancer cells. Interestingly, miR-214 targets protein tyrosine kinase 6 (PTK6) and reduced PTK6 expression. The restoration of PTK6 expression significantly blocked the inhibitory effect of miR-214 on cell proliferation. Moreover, inactivation of PKT6 by ibrutinib and expression of miR-214 significantly enhanced the cell death in prostate cancer cells. Collectively the data suggest that miR-214 functions as a tumor suppressor in prostate cancer, and may become a potential therapeutic target in prostate cancer. Citation Format: Patrice Cagle, Suryakant Niture, Anvesha Srivastava, Malathi Ramalinga, Leslimar Rios-Colon, Uchechukwu Chimeh, Deepak Kumar. MicroRNA-214 inhibits prostate cancer cell proliferation, migration, invasion and increases drug sensitivity by targeting PTK6 [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2019; 2019 Mar 29-Apr 3; Atlanta, GA. Philadelphia (PA): AACR; Cancer Res 2019;79(13 Suppl):Abstract nr 3561.