Abstract 3544: The gastric mucosal tissue interleukin-8 mRNA expression level is a promising prognostic marker for prognosis prediction in advanced gastric cancer treatment.

Abstract

Abstract Background : The Interleukin-8 is one of the potent chemokine that plays the major role in cancer inflammatory and vascular growth pathway. We conduct this experiment to determine how difference of its level between non-cancer and cancer location, and its possibility to predict prognosis and result of advance gastric cancer treatment . Method : We collected 309 gastric tissue samples from 129 Thai advance gastric cancer patients during January 2007 to March 2012 . Clinical information was prospective collected on electronic data base. The Interleukin-8 expression levels were detected by relative quantitation RT-PCR method with Taqman probe for Interleukin-8 on 7500 series machine, Applied Biosystem . Test of difference of Mean was done with independent and paired t-test by SPSS version 17th. Test of difference was considered significant by p value ≤0.05. Result : The gastric mucosal tissue IL-8 mRNA expression level was significantly higher in tumor specimen than benign specimen by level of 5469.35 and 565.42 respectively (p =0.13). Its level in diffuse histologic type,metastatic disease and positive lymph node group are higher without significant difference. In subgroup analysis for 50 neo- adjuvant chemotherapy patients, the responder group (partial response and complete response) had a lower IL-8 expression level than non-responder group by the means level of1904.02, 24269.31,respectively (p=0.209). Conclusion : Gastric mucosal tissue IL-8 mRNA expression level was remarkable increased in advance gastric cancer and signet ring cell type. We found that the higher expression level is directly correlated with more advance of histological grading and prognosis of disease treatment. The chemotherapy response rate was poorer in extremely high expression. High interleukin-8 expression could be considered as poor clinical prognostic factor for gastric cancer treatment, and it may be helpful tool to predict the chemotherapy response in future. Citation Format: Bandhuphat Chakrabandhu. The gastric mucosal tissue interleukin-8 mRNA expression level is a promising prognostic marker for prognosis prediction in advanced gastric cancer treatment. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 3544. doi:10.1158/1538-7445.AM2013-3544