Abstract
Abstract Colorectal cancer (CRC) is the third most common type of cancer worldwide and the second most common cause of cancer related deaths in Europe. Since Countries of Central Europe (Czech Republic, Slovakia, Hungary) have one of the highest rates both for incidence and mortality of CRC, CRC became a serious health, social and economic problem. CRC pathogenesis involves multiple genetic events. On behalf of a strong experimental work, there is still lack of predictive, prognostic and treatment-response related biomarkers. Long non-coding RNAs (lncRNA) are non-protein coding transcripts longer than 200 nucleotides. Deregulation of lncRNAs is often seen in a variety of human diseases, including cancer. Taken together, this makes lncRNAs the potential regulators of cancer progression, therapeutic targets or markers of treatment efficacy. The main aim of our present study was to define expression patterns of lncRNAs in colorectal tumor tissue and define their role in cancer cell proliferation, survival and DNA damage response (DDR). In the first part of our study, we analyzed the expression profile of 83 lncRNA known to be associated with cancer progression in cohort of CRC tumor tissues and adjacent mucosa. We found 12 upregulated lncRNAs (p<0.05) and 10 downregulated lncRNAs (p<0.05). Our results have been replicated on larger set of patients (100 CRC patients) and those lncRNAs associated with cell proliferation, survival and DNA damage response are functionally tested on colorectal cell lines (HCT116, HCT116 p53-/-) in vitro. Definition of role of lncRNA as a tumor suppressors or oncogenes in CRC etiology may lead to better understanding of regulation of CRC carcinogenesis. Furthermore, understanding the role of lncRNA in DDR may contribute to better treatment response of CRC patients and to define markers of individual treatment response. Acknowledgement: GACR 15-14789S, AZV 15-27580A, GAUK800216, AZV 15-26535A, SUSCEPTCOST LD14050 Citation Format: Alena Opattova, Fábio Miguel Ferreira, Jozef Horak, Sona Vodenkova, Pavel Vodicka. Long non-coding RNAs in colorectal cancer [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2017; 2017 Apr 1-5; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2017;77(13 Suppl):Abstract nr 3496. doi:10.1158/1538-7445.AM2017-3496
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