Abstract 3488: HPV detection by PCR in real time in peruvian women

Abstract

Abstract Background. In Perú Cervical cancer is a significant public health problem, according Globocan, it is the most frequent cancer among women in Peru, the incidence of this cancer is 34.5 and the mortality 16.3. In 2004, cervical cancer was responsible of 66,771 DALYs in Peru, 18.2% of the total. The human papillomavirus (HPV) is a well-known cause of cervical cancer. HPV tests are used as an adjunct test to decrease the false-negative rate of cytological screening. The HPV test will permit to separate the population in two groups, women with high risk to cancer cervical (HPV+) and women with low risk to cancer cervical (HPV-). Objective. To identify the prevalence of the genotypes of Human Papillomavirus (HPV) in Peruvian women from marginal urban area of Lima according age. Methodology. Samples of 5460 women aged 18 to 63 years were studied for analysis by the automated Roche Cobas 4800 system that detects genotypes 16 and 18 specifically and 12 other genotypes (31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 68, 66) considered high risk HPV, for this purpose the system uses specific primers for the L1 region of the viral genome and control the system using B globin gene. Results and Conclusions. According PCR screening the total samples were 5,460 (100%), 855 (15.62%) were positive by PCR, of the PCR positive 319 were positive by CBL (5.84%), 16 of them were LIE-AG (0.29%) and 0 carcinomas. The genotypes of PVH more frequent was 16 (16%), genotype 16 plus others high risk virus was 69 (8%), PVH 18 was 8 (3%). Of the 853 women that were positive for PCR, 149 of them had the highest risk HPV genotype 16 and the age group that most often present for this genotype was 30 to 39 years, this group is consider as high risk population. In general, PCR have been demonstrated an adequate prevalence rate of women in high risk according of other literature and we consider as an important test to cervical cancer control. Ilustración 1. Frequency of HPV in Peruvian women. Illustration 2. HPV Genotypes in peruvian women Illustration 3. Distribution of CBL (+) Ilustración 3. Genotipos de PVH y distribución por grupo etáreo. / HPV genotype distribution per age group. Citation Format: Javier Manrique, Gustavo Sarria, Abelardo Arias, Pamela Mora, Abel Limache, Maria del Carmen Nuñez, Yasser Sullcahuaman, Tatiana Vidaurre. HPV detection by PCR in real time in peruvian women. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 3488. doi:10.1158/1538-7445.AM2014-3488