Abstract 3323: Breast cancer stem cell culture and enrichment using poly(ϵ-caprolactone) 3D scaffolds

Abstract

Abstract Introduction: Cancer stem cell (CSC) population displays self-renewal capabilities, resistance to conventional therapies and a tendency for post-treatment recurrence. Increasing knowledge about CSCs phenotype and functions is needed to investigate new therapeutic strategies against CSC population. Poly(ϵ-CaproLactone) (PCL) as biocompatible and free toxic dye polymer, have been used to manufacture scaffolds, solid structures suitable for 3D cancer cell culture. It has been described that scaffold cell culture enhances the CSCs population. Experimental procedures: A RepRap BCN3D+ printer and 3.5 mm PCL wire were used to fabricate circular scaffolds. PCL design and fabrication parameters were first determined and then optimized considering several product features of the resulting scaffolds. MCF7 breast carcinoma cell line was used to assess scaffolds adequacy for 3D cell culture. To evaluate CSC enrichment the Mammosphere Forming Index (MFI) was performed in 2D and 3D MCF7 cultures. Summary of the new, unpublished data: Results shown that the 60º scaffolds were more suitable for 3D culture than the 45º and 90º ones. Moreover, 3D culture experiments both, in adherent and non-adherent conditions, showed a significant increase on MFI compared to 2D cultures (control). Thus, 3D cell culture with PCL scaffolds could be useful to improve cancer cell culture and to enrich the CSCs population. Basically cells population growth with a 3D culture better and higher in value as it is demonstrated within the work. Conclusions: Thus, 3D cell culture with PCL scaffolds could be useful to improve cancer cell culture and to enrich the CSCs population for further pre-clinical studies on CSC molecular characterization and treatment. Citation Format: Marc Rabionet, Sonia Palomeras, Ines Ferrer, Ariadna Sarrats, Ariadna Giro-Perafita, Maria Luisa Garcia-Romeu, Joaquim Ciurana, Teresa Puig. Breast cancer stem cell culture and enrichment using poly(ϵ-caprolactone) 3D scaffolds. [abstract]. In: Proceedings of the 107th Annual Meeting of the American Association for Cancer Research; 2016 Apr 16-20; New Orleans, LA. Philadelphia (PA): AACR; Cancer Res 2016;76(14 Suppl):Abstract nr 3323.