Abstract 331: SIRPB1 promotes prostate cancer cell proliferation & migration

Abstract

Abstract Signal-regulatory-protein beta 1(SIRPB1) is a member of the signal-regulatory-protein (SIRP) family that belongs to the immunoglobulin superfamily and is capable of modulating receptor tyrosine kinase-coupled signaling. The copy-number variations (CNV) at the SIRPB1 locus were associated with aggressiveness of prostate cancer in patients. To test if SIRPB1 could affect prostate cancer development and progression, we investigated the potential role and mechanisms of SIRPB1 action in prostate cancer cell lines in vitro and in xenograft tumors. Knockdown of SIRPRB1 by RNA interference resulted in significant suppression of cell colony formation, cell mobility, cell migration, and invasion. The knockdown also induced cell cycle arrest during the G0/G1 phase and a remarkable enhancement of apoptosis in PC3 prostate cancer cells. In contrast, overexpression of SIPRB1 significantly induced cell migration, invasion, colony formation and cell cycle in C4-2 prostate cancer cells. Furthermore, overexpression of SIRPB1 in C4-2 cell model enhanced its tumor take rate in nude mice. Also, SIRPB1 mRNA expression was increased in up to 39% of the prostate cancer specimens based on in silico analysis of several public databases. These results suggest that SIRPB1 is a potential oncogene in the prostate and could be used as a biomarker to identify patients at risk of developing aggressive prostate cancer. Citation Format: Qiong Song, Siyuan Qin, Chunlin Zou, Wenchu Wang, Lihui Wang, Haibo Tong, William J. Catalona, Jian Zhang, Yi Lu, Zhou Wang. SIRPB1 promotes prostate cancer cell proliferation & migration [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2017; 2017 Apr 1-5; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2017;77(13 Suppl):Abstract nr 331. doi:10.1158/1538-7445.AM2017-331