Abstract
Abstract Colorectal cancer is the 3rd most common cause of cancer-related deaths for men and women in the United States. Understanding the mechanisms associated with the initiation and progression of colorectal cancer is needed to develop precise therapies. Multiple regulatory processes are responsible for maintaining cellular homeostasis, including microRNA (miRNA) regulation. We previously demonstrated that miR-137 expression was significantly decreased in 84% of tumors as compared to adjacent mucosal tissue from patients with rectal cancer (n = 68). Restoration of miR-137 reduced colon cancer growth, measured by colony formation and tumorsphere assays. Induction of miR-137 expression significantly decreased tumor growth in our xenograft tumor model by approximately 50% (n = 10). To understand the mechanism associated with the tumor suppressive function of miR-137, we transfected miR-137 mimic into HCT-116 cells and performed microarray analysis to identify changes in gene expression and identify key regulatory pathways effected by miR-137 restoration. Multiple miR-137 targets were down regulated, as compared to cells treated with a negative control mimic. The data was validated using quantitative real-time PCR (qPCR). Finally, the expression of miR-137 target genes was analyzed in a panel of colon cancer cell lines using qPCR. In summary, miR-137 acts as a tumor suppressive microRNA by negatively regulating multiple targets that are overexpressed in colorectal cancer. Citation Format: Amber Rae Smith, Rebecca Marquez, Bryan Tsao, Alexandria Roy, Bailey Wilkerson, Surajit Pathak, Kristi Neufeld, Xiao-Feng Sun, Liang Xu. Small but lethal, miR-137 acts as a tumor suppressive microRNA in colorectal cancer. [abstract]. In: Proceedings of the 106th Annual Meeting of the American Association for Cancer Research; 2015 Apr 18-22; Philadelphia, PA. Philadelphia (PA): AACR; Cancer Res 2015;75(15 Suppl):Abstract nr 3127. doi:10.1158/1538-7445.AM2015-3127
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