Abstract 3094: Identification of mimotopes of the epithelial mucin-1 (MUC1) in non-small cell lung cancer

Abstract

Abstract Background Mimotope analysis has been widely used in mapping epitopes for drug target, and also used in the development of new diagnostics, therapeutics and vaccines. The epithelial mucin-1 (MUC1) is a glycoprotein in human endometrium and has been found highly expressed in tumor tissues of NSCLC patients. Mimotopes of MUC1 has great potentials to be immunotherapy targets for NSCLC. In this study, we use phage display technology to screen a T7 NSCLC phage library to identify mimotope peptides of MUC1 proteins. Method A T7 NSCLC phage library was screened by 3-round of biopanning with anti-MUC1 monoclonal antibody acting as a molecular target. Forty positive clones were picked for ELISA confirmation using anti-MUC1 antibody. In addition, these clones were then amplified and coated with ELISA plates. Forty NSCLC patient and 40 control serum samples were used to test the ELISA plates. Statistical analysis was preformed, and the most significant clones were picked and PCR amplified for sequencing. Result Two peptide sequences, AAPDFRP and SAPDDRP, were identified and the competitive inhibitory rates of MUC1 were more than 50%. The intensity between MUC1 monoclonal antibody and the positive clones of these two sequences had positive correlation. In addition, in serum binding specificity detection, these two amino acid sequences showed higher tumor binding specificity with lung cancer patients serum than that of healthy human serum(P<0.05). Conclusion Peptides of AAPDFRP and SAPDDRP showed a high homology in the mimic epitopes of the epithelial mucin-1 (MUC1) glycoprotein in NSCLC. These may be potential immunotherapy targets which could also act as candidates of tumor vaccines Citation Format: Yu Qiu, Wang Jianfei, Cao Ruoqiong, Zhang Yi, Sun Yangyang, Li Zhong. Identification of mimotopes of the epithelial mucin-1 (MUC1) in non-small cell lung cancer. [abstract]. In: Proceedings of the 107th Annual Meeting of the American Association for Cancer Research; 2016 Apr 16-20; New Orleans, LA. Philadelphia (PA): AACR; Cancer Res 2016;76(14 Suppl):Abstract nr 3094.