Abstract 3091: Oncogenic roles of nuclear VCP in oral cancer development

Abstract

Abstract VCP is a member of AAA-ATPase family that includes putative ATP-binding proteins involved in nuclear envelope reconstruction, cell cycle regulation, Golgi reassembly, suppression of apoptosis, and DNA-damage response. Due to the novel functions, VCP overexpression was linked to cancer development and suggested as a prognostic tumor marker for poor clinical outcome. Our preliminary data of SNP array analysis on 33 fresh clinical OSCC samples identified an amplified region at chromosome 9p13.3 containing the VCP gene. This finding was further validated by FISH analysis on a larger scale using paraffin-embedded oral cancer tissues. Consistent with genomic copy alterations, the mRNA and protein expression levels of VCP were also found higher in OSCC tissues. Interestingly, the nuclear VCP staining correlates with VCP gene amplification and shorter overall survival, suggesting the novel functions of VCP in nucleus during OSCC development. In this study, we identified several nuclear binding partners for VCP in OSCC cells, and signaling networks controlled by VCP during OSCC development were discussed. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 3091.