Abstract 3046: Association of XRCC1 Arg194Trp polymorphism and XPD Lys751Gln polymorphism with biochemical parameters, severity and risk of prostate cancer in Bangladesh Population

Abstract

Abstract a)To evaluate the association between x-ray cross-complementing gene 1 (XRCC1) polymorphism Arg194Gln and xeroderma pigmentosum group D (XPD) polymorphism Lys751Gln with severity and risk of prostate cancer as well as with biochemical parameters of prostate cancer patients in Bangladesh population. b) Blood samples and data were collected from 132 prostate cancer patients and 135 healthy controls among Bangladesh population. Genotype analysis was done by the PCR-RFLP method. Statistical analysis was performed using GraphPad Prism 9.4.1 software. c) Only the recessive model, that is, carrying two copies of the XRCC1 194 codon Trp allele was positively associated with prostate cancer, with an OR of 7.50 (95% CI, 1.227-85.12; p=0.035). No association was found between the XPD variants with prostate cancer risk. Keeping the wild type and heterozygous genotype as a reference, the combined effects of the XRCC1 codon 194 Arg/Arg and the XPD codon 751 Gln/Gln genotype were found to be significantly associated with prostate cancer risk with an OR of 3.25 (95% CI, 1.39-8.20; p=0.006). XRCC1 194 Trp/Trp genotype was significantly associated with obstructive features, such as hematuria and creatinine levels of the prostate cancer patients (P<0.05). Moreover, the homozygous mutant Gln/Gln genotype of XPD gene was also significantly associated with creatinine levels in prostate cancer patients. No association was found between any of the variants of XRCC1 and XPD with invasiveness, tumor grade, prostate ultrasound scan results, digital rectal examination, Urine R/E or Prostate Specific Antigen (PSA) results (P>0.05). d) Only the XRCC1 codon 194 Trp allele showed a modest association with prostate cancer risk with an 8.19-fold increased risk when two copies of the allele were present. When both alleles- XRCC1 codon 194 and XPD codon 751 Gln -were present in their homozygous states, the risk for prostate cancer increased by 3.2-folds. Our results showed a significant association between XRCC1 194 Trp/Trp with obstructive features and and levels of creatinine in prostate cancer patients of Bangladeshi men. Citation Format: Nafisa Ahmed, MD. Ariful Islam, M. Mahboob Hossain, Yearul Kabir. Association of XRCC1 Arg194Trp polymorphism and XPD Lys751Gln polymorphism with biochemical parameters, severity and risk of prostate cancer in Bangladesh Population [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2023; Part 1 (Regular and Invited Abstracts); 2023 Apr 14-19; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2023;83(7_Suppl):Abstract nr 3046.