Abstract 304: Reactive oxygen species induce prostate cancer cells via activation of the IL6/STAT3 pathway.

Abstract

Abstract Background: Previously we have established a stepwise prostate carcinogenesis model including human primary prostate epithelial EP156T cells, non-malignant mesenchymal EPT1 cells and pre-malignant EPT2-D5 cells. Aims and methods: To further achieve malignant cells, EPT2-D5 cells were grown in protein-free medium and the tumorigenesis was tested in vivo. Results: 1) Lots of spheres were generated in monolayer culture of D5 cells in protein free medium. The sphere forming cells were named D5HS. 2) Different from EPT2-D5 cells, D5HS formed large subcutaneous tumors (EPT3) and metastasis (EPT3M) in SCID mice. 3) Gene expression profiling showed inflammation signatures in D5HS. 4) Cellular Reactive Oxygen Species Detection Assay (DCFDA) showed high reactive oxygen species (ROS) in D5HS, and ELISA and Western blotting showed high secreted IL6 and high STAT3 expression in D5HS. 5) Blocking of ROS or STAT3 signaling reduced the sphere formation. 6) Immunohistochemistry staining showed high pSTAT3 level in the EPT3 tumor. Conclusions: Reactive oxygen species induce prostate cancer cells via activation of the IL6/STAT3 pathway. Citation Format: Yi Qu, Runhui Liu, Jigang Zhang, Mihaela Popa, Yaping Hua, Biaoyang Lin, Emmet McCormack, Weidong Zhang, Anne Margrete Oyan, Karl-Henning Kalland, Xisong Ke. Reactive oxygen species induce prostate cancer cells via activation of the IL6/STAT3 pathway. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 304. doi:10.1158/1538-7445.AM2013-304