Abstract 2931: The compounding effects of coenzyme q10 and radiation treatment on glial fibrillary acidic protein network of glioma in vitro

Abstract

Abstract Introduction: Astrocytoma is an aggressive primary malignant brain tumor in adults. Radiation therapy is an effective medical treatment, but one of its undesired side-effects is the induced genomic instability of surrounding astrocytes, which can lead abnormal distribution of GFAP and associates with severe brain injury (BI). Purpose: In order to cope with this serious difficulty, various antioxidants have been applied with radiation therapy. Among antioxidants, Coenzyme Q10 (Q10) is one of the few candidates without double-edged sword effect. However, the understand about Q10 therapeutic effect on radiological protection is limited. In this study, we aimed to investigate the potential therapeutic effects of Q10 on brain tumor radiation treatment. Materials and Methods: Astrocyte and glioma cell lines were cultivated with Q10 (10μM~ 50 μM) and exposed to 10 Gy single dose from Cobalt-60 (60Co). After the radiation exposure, cell viability was analyzed using MTT assay, GFAP responses was examined by immunofluorescence staining and qPCR, and other cell phenotype indexes, such as the morphology, migration, and invasion in vitro, were observed by microscopy. Results: Our results indicated that Q10 treatment was capable of maintaining GFAP network of astrocyte after radiation exposure. Besides, Q10 synergistically eliminated glioma proliferation after radiation treatment. By extending horizontally along the base of GFAP remodeling, we further investigated the ability of 3D tumor invasion in co-culture model which simulated the microenvironment composed of reactive astrocyte and glioma in clinical research. Results indicated that radiation therapy with Q10 can further suppress the invasive ability in vitro. Conclusions: In sum, our results demonstrated the potential radiological protection of Q10 on astrocyte and amplified the therapeutic effectiveness for brain tumor therapy. Citation Format: Chong-Chun Liao, Megha Jhunjhunwala, Li-Han Chung, Chi-Shuo Chen. The compounding effects of coenzyme q10 and radiation treatment on glial fibrillary acidic protein network of glioma in vitro [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2019; 2019 Mar 29-Apr 3; Atlanta, GA. Philadelphia (PA): AACR; Cancer Res 2019;79(13 Suppl):Abstract nr 2931.