Abstract
Abstract Non-thermal atmospheric pressure biocompatible plasma (NBP) has been reported that was possible to induce apoptosis on various cancer cells including lung, ovary, brain, liver, breast, and pancreas cancer. We developed NBP device, which has been reported to have cytotoxicity on cancer cells induce DNA damage and apoptosis. In particular,reactive oxygen and/or nitrogen species (RONS) were generated by plasma device to give oxidative stress and depolarization of mitochondria membrane in cancer cells. Now we developed a microwave plasma jet system, which could generate nitric oxide (NO) and NO-plasma activated water (NO-PAW). Previously, we found that NO-PAW could induce apoptosis and accumulate ROS in human cervical cancer cells (1). In this study we investigated the efficacy of NO-PAW on oral squamous cancer cells (OSCC), a common malignant tumor of the head and neck (2, 3). We observed that cytotoxicity and apoptotic stimulation by NO-PAW were significantly increased. Also, expression level of caspase and PARP in OSCC was increased after treatment by NO-PAW. However, the apoptosis effect was not observed in human gingival fibroblasts (as normal human cells). Additionally, we found that NO-PAW inhibited cancer progression in vivo. In conclusion, NO-PAW could suppress the progress of OSCC by inducing cell apoptosis. This indicated NO-PAW as a novel tool for OSCC therapy, and probably will be also effective for other oral cancer. Acknowledgments: This work was supported by the Leading Foreign Research Institute Recruitment Program through the National Research Foundation of Korea (NRF- 2016K1A4A3914113) funded by the Ministry of Science, ICT, and Future Planning (MSIP) of the Korean Government for EH. Choi, I. Han. The Korea government, and the Basic Science Research Program through the NRF of Korea, funded by the Ministry of Education (NRF-2015R1C1A2A01054137) for I. Han.
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