Abstract 2793: Application of conditionally reprogrammed cell in in vitro drug sensitivity test and PDX modeling

Abstract

Abstract Tumor cell immortalization through conditional reprogramming, particularly from limited biopsy specimen, is an invaluable tool to generate propagating tumor cells for cell-based drug sensitivity assay and bio-banking in vitro. We have successfully reprogrammed primary tumor cells from a number of tumor types, including lung carcinoma, breast cancer and glioma .etc. The conditionally reprogrammed cells exhibited typical colonized growth, which is well maintained upon cryopreservation. In some cases, the cells can be passaged for multiple times and likely become sable cell lines. Like primary tumor cells, conditional reprogrammed tumor cells are reliable to test drug sensitivity in vitro. We attempt to extend conditional reprogrammed tumor cells to reconstitute and grow PDX models in vivo for testing drug efficacy. The conditional reprogrammed tumor cells could also be used in PDX establishment and the tumor formation rate is much higher than the primary tissue's. In summary, this study explores and expands application of conditional reprogrammed tumor cells in in vitro drug efficacy test and in vivo PDX Modeling. Citation Format: Yuan Long, Song Xi, Danyi Wen. Application of conditionally reprogrammed cell in in vitro drug sensitivity test and PDX modeling [abstract]. In: Proceedings of the Annual Meeting of the American Association for Cancer Research 2020; 2020 Apr 27-28 and Jun 22-24. Philadelphia (PA): AACR; Cancer Res 2020;80(16 Suppl):Abstract nr 2793.