Abstract 2787: PrimePanel provides a high throughput in vitro drug screening platform that intimately links to in vivo pharmacological analysis in PDX models.

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Abstract Patient derived xenografts (PDX) are in vivo animal tumor models established directly from patient tumor samples without any in vitro manipulation. It has been shown that these tumor xenografts maintain essential histopathological features and genetic profiles of the original tumors, and thus are the most clinically relevant animal models for cancer drug discovery. Comparing to the traditional cell line derived xenograft (CDX) tumor models, however, one challenge encountered by the PDX in vivo studies is the lack of corresponding in vitro cell culture system for cost-effective and high throughput drug screening and model selection. Here we set out to establish such an in vitro platform, so-called PrimePanelTM, by deriving homogeneous primary cancer cell cultures from the PDX tumors in several major tumor types. These cells are early passage cultures (usually p<10), which maintain similar cellular characteristics as the corresponding tumor xenografts, including the genomic mutational status, biochemical signaling, and responses to tumor cell autonomously targeted therapeutics. Thus, PrimePanelTM offers a higher throughput, faster turnaround, more versatile and lower cost platform to assess drug efficacy prior to in vivo study, which could fulfill a critical need in early stage drug discovery. Citation Format: Yanxia Zhang, Yuqiang Ge, Yanan Liu, Jing Zhao, Lanhua Li, Meng Qiao, Jinying Ning, Jiping Zha, Guizhong Liu. PrimePanel provides a high throughput in vitro drug screening platform that intimately links to in vivo pharmacological analysis in PDX models. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 2787. doi:10.1158/1538-7445.AM2013-2787 Note: This abstract was not presented at the AACR Annual Meeting 2013 because the presenter was unable to attend.