Abstract 2482: Effect of L-isoleucine and L-leucine at early stage of rat bladder carcinogenesis

Abstract

Abstract We previously demonstrated the promotion effects of L-isoleucine and L-leucine on N-butyl-N-(4-hydroxybutyl)nitrosamine (BBN)-induced rat bladder carcinogenesis when supplemented in AIN-93G diet in a 29-week bioassay. However, little is known about the mechanism underlying the promotion effects. The aim of the present study is to evaluate the effects of L-isoleucine and L-leucine at early stage of rat bladder carcinogenesis. In experiment 1, rats were fed AIN-93G basal diet, 2% L-leucine or L-isoleucine in basal diet for 2 weeks. In experiment 2, rats were administered 0.05% BBN in drinking water for the first 2 weeks, and then fed basal diet, 2% L-leucine or L-isoleucine diet for 4 weeks. No treatment-related histopathologic changes in bladder were observed in both experiments. There were also no differences in proliferative activities and expressions of 12 genes that were previously found to be up-regulated in BBN-treated bladder urothelium from week 2 between basal diet group and L-leucine or L-isoleucine group regardless of BBN initiation. These findings indicated the possibility of lack of effects of L-isoleucine and L-leucine at early stage of rat bladder carcinogenesis. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 2482.