Abstract 2468: Increased expression of snoRNA in the tumor vasculature

Abstract

Abstract The tumor microenvironment has long been known to cause the differential expression of many genes within the endothelial cells that comprise the tumor vasculature. Yet, the differential expression of short nuclear RNA (snoRNA) has not previously been observed within tumor associated endothelial cells. A differential gene expression analysis was conducted on total RNAseq data derived from primary endothelial cell isolates and validated by qPCR. By these means, SNORD75, SNORD76, SCARNA7 and SNORA81 were demonstrated to be expressed at a higher level in liver, lung and colon associated tumor endothelial cells when compared to patient matched endothelial cells from healthy adjacent tissue. This data suggests that these four snoRNA molecules could be contributing to pathological angiogenesis and therefore tumor growth. Further work is currently being conducted to investigate this possibility. Citation Format: Klarke M. Sample, Yingjie Nie, Roy Bicknell. Increased expression of snoRNA in the tumor vasculature [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2018; 2018 Apr 14-18; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2018;78(13 Suppl):Abstract nr 2468.