Abstract 2424: Conditioned media enhances proliferation and mobility in triple negative breast cancer cells

Abstract

Abstract Background. Triple negative breast cancer (TNBC) is one of the most aggressive forms of breast cancer and disproportionally affects African American women. This subtype of breast cancer is characterized by the absence of the estrogen, progesterone, and HER2/neu receptors. Our research has provided insight on understanding that the microenvironment, specifically noncancerous cells, play in enhancing cancer progression and metastasis. However, the role of adjacent noncancerous cells in the metastatic phenomenon is poorly understood. Therefore, the aims of this project were: 1) to determine if conditioned media from MCF-10A cells would enhance proliferation of MDA-MB-231 and MDA-MB-468 cells and 2) to evaluate the ability of the effects of migration of the conditioned media on the MDA-MB-231 and MDA-MB-468 cells. Methods. Crystal violet and alamar blue dye exclusion assays were performed to measure proliferation in the different medias. The trans well migration assay was used to evaluate motility enhancement based on the media used, either conditioned media and the Cellometer was used to evaluate whether the MDA-MB-468 and MCF-10A cells shared the same microenvironment. Results. Conditioned media is made from base medium with a concentration of 1.0 g/L glucose with .5% FBS exposed to non-cancerous MCF-10A cells that have been driven into crisis. This conditioned media encouraged robust and continuous proliferation of the tumorigenic MDA-MB-231 and MDA-MB-468 cell lines. HGCM was extracted in our lab using sterile technique and reused to analyze the proliferative effects on cancerous and non-cancerous cells. MDA-MB-231 cells demonstrated significant proliferation when compared to the control. MDA-MB-468 cells demonstrated a similar response. The trans well migration assay demonstrated that the GFP labeled MDA-MB-468 cells had a higher percentage of motility than with MCF-10A cells in standard media. Conclusions: Taken together, these data indicate that factors secreted into the microenvironment from noncancerous cells may contribute to the mobility of TNBC cells and the conditioned media contains unknown factors that promote the cell mobility of the MDA-MB-231 and MDA-MB-468 cells. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 2424. doi:1538-7445.AM2012-2424