Abstract
Abstract We have developed a novel technique to orthotopically transplant rectal cancer cells. This model was established by intrarectal transplantation of mouse rectal cancer cells stably expressing fluorescent protein, followed by disrupting the epithelial cell layer of the rectal mucosa by soaking with an acetic acid solution. Early stage tumor was detected on the rectal mucosa from as early as 6 days after transplantation, which then became invasive into the submucosal tissue. The tumor incidence (%) on the rectal mucosa and size (mean volume ± SD) were 100% and 1232.4 ± 994.7 mm3 at 4 weeks after transplantation, respectively. Spontaneous lymph node metastasis and lung metastasis were also observed 4 weeks after transplantation in over 90% of mice. This rectal tumor model to mimic of the natural course of rectal cancer accurately. The model will be more useful for evaluation of the tumor environment, metastasis and agents which suppress or enhance the growth of tumor at early stages as well as metastasis. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 2409. doi:10.1158/1538-7445.AM2011-2409
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