Abstract 2352: Place of birth influences the metabolomic profile and preliminary effectiveness of a weight loss intervention among Hispanic men from the ANIMO pilot study

Abstract

Abstract Background: The effect of intrinsic and extrinsic factors (e.g. the environment and genetics) on the metabolome may have implications for disease risk, health disparities, and effectiveness of clinical interventions. Purpose: This study investigated whether plasma metabolomic profiles differ between U.S. born and foreign-born Hispanics participating in a weight loss intervention. Secondarily, we sought to examine if country of origin had an effect on the intervention effectiveness. Methods: Participants were overweight and obese Hispanic men (N=42) aged 18 to 64 years enrolled in a 24-week weight loss intervention. Metabolomic profiles were acquired from plasma using ultra performance liquid chromatography-mass spectroscopy (UPLC-MS) at baseline, 12 and 24 weeks. Models were visualized using supervised orthogonal projections to latent structures-discriminant analysis (OPLS-DA); R2X cut points of 0.75, 0.50, and 0.25 were used to describe substantial, moderate, or weak levels of predictive accuracy, respectively. Score plots (S-plots) were utilized to select metabolites that influenced the OPLS-DA model. Progenesis QI was used for peak integration and metabolite identification in addition to the Human Metabolite Data Base (HMDB). The effect of place of birth on weight loss was examined using multiple linear regression. Results: Foreign-born participants were older (P = 0.0086), had less education (P < 0.001), were mostly Spanish-preferred speakers (P < 0.001), and had less acculturation (P < 0.001) compared to U.S. born participants. The metabolome between U.S. born vs foreign-born were moderately (R2X =0.65), weakly (R2X= 0.33), and substantially (R2X =0.79) different at baseline, 12 weeks, and 24 weeks. Endogenous metabolites such as alpha-keto and amino acids, phospholipids, fatty acids, as well as some dietary compounds were characteristic of the foreign-born participants, but not the U.S. born, at baseline. Post-intervention, metabolites from dietary sources (cereals, herbs and spices, mushrooms, chives, onions, and beer), and lipids (diacylglycerols, cholesteryl and glycerolipids) where characteristic of the foreign-born group, whereas saponins and metabolites from green vegetables, avocados, alcoholic beverages, coffee, and green tea were characteristic of the U.S. born group. Models comparing study arm or weight loss status were weak. At 24 weeks, U.S. born participants had more weight loss than foreign born (P = 0.023). Conclusion: Plasma metabolomic profiles from Hispanic men differ by country of origin. Analyses to determine whether the greater intervention benefit among U.S. born Hispanic males can be explained by their baseline metabolome are pending. Future studies should determine factors that influence differences in metabolomic profiles in order to tailor interventions. Citation Format: Melissa Lopez-Pentecost, David O. Garcia, Xiaoxiao Sun, Cynthia A. Thomson, H-H. Sherry Chow, Wade M. Chew, Jessica A. Martinez. Place of birth influences the metabolomic profile and preliminary effectiveness of a weight loss intervention among Hispanic men from the ANIMO pilot study [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2021; 2021 Apr 10-15 and May 17-21. Philadelphia (PA): AACR; Cancer Res 2021;81(13_Suppl):Abstract nr 2352.