Abstract
Abstract HDGF was identified as an oncogene relevant for colon cancer carcinogenesis by cDNA-array analysis, RT-PCR and immunohistochemistry. The analysis were performed in colonic adenoma (Geki2), carcinoma (HT29) and metastasis (SW620) cell lines, and in 50 colorectal cancer tissues and corresponding normal mucosa. By incubation of the cell lines with the short chain fatty acid butyrate (4mM), it was possible to (down-) regulate the HDGF-expression level. In the patients we analysed, HDGF overexpression was not associated with a worse prognosis or any other clinical parameter. To study the mechanisms of action of HDGF we stably transfected a HDGF-siRNA containing plasmid into HT29 colon carcinoma cells, resulting in a almost complete inactivation of HDGF-expression. This blocking leads to altered growth characteristics and cell differentiation and the cells exhibit potent pro-apoptotic properties. An affymetrix-expression array was performed and first results will me shown at the meeting. In conclusion, HDGF might be a potential therapeutic target for human colorectal cancer and a modulation of expression is possible by the short chain fatty acid butyrate. These findings may have major implications in the treatment of colorectal cancer. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 2248. doi:10.1158/1538-7445.AM2011-2248
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
