Abstract 2219: Expression of PCNA and MCM2 in ameloblastoma

Abstract

Abstract Background:-Ameloblastoma is an odontogenic neoplasm with the most frequent osseous destructive lesions of the jaws. Ameloblastoma deserves special attention, because of its biological behaviour exhibiting great infiltrative potential, high recurrence rate and capacity to metastasize. The increased proliferation rate in the odontogenic epithelium can be a result of perturbations in the cell cycle regulators and dictates the aggressive behavior of the tumors. Investigating proliferative rates in the epithelial lining of these lesions helps in assessing their biological behaviour. Various cell proliferation markers are used as diagnostic and prognostic tools in oral lesions. Assessing the expression of these markers helps in estimating the proliferative index of the tissues. Aim:- The aim of this study is to analyze the presence of, Proliferating cell nuclear antigen (PCNA) and the minichromosome maintenance-2 (MCM2) proteins, two well established markers of cell proliferation, in clinically diagnosed cases of ameloblastoma and correlate with aggressiveness and its types (Solid/multicystc{Follicular, plexiform} unicystic). Materials and method:-14 clinically diagnosed cases of ameloblastomas (10 Solid/multicystic cases{6 cases of Follicular, 4 cases of plexiform} and 4 cases of unicystic) are obtained from the archieves of the Department of Oral and Maxillofacial Pathology to study the expression of MCM2 and PCNA. The data was analysed by Kruskals ANOVA, Mann-Whitney U test and Post hoc analysis. Results and conclusion:- A Kruskal-Wallis ANOVA was used to detect if there was a significant difference in the expression of MCM2 and PCNA between the three variants of ameloblastoma. There was a significant difference between the three groups for mean percentage expression of MCM2 but there was uniformly higher expression of PCNA in all the three variants. Interestingly, further post hoc analysis revealed that the expression of MCM2 was significantly higher in the follicular variantas compared to the unicystic variant, suggesting that dual staining of MCM2 and PCNA may be a better marker for follicular variant. Keywords:-Ameloblastoma, MCM2, PCNA. Citation Format: Bhavana Mallipudi. Expression of PCNA and MCM2 in ameloblastoma [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2018; 2018 Apr 14-18; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2018;78(13 Suppl):Abstract nr 2219.