Abstract 2208: JNK2 regulates the hif-1α mRNA stability in a nucleolin-dependent manner

Abstract

Abstract HIF-1α mRNA is regarded as constitutively and ubiquitously expressed no matter the level of oxygen tension. But most recently, more and more labs found that HIF-1α mRNA could be regulated by natural antisense transcripts, potential microRNAs, and low O2 pressure in the renal medulla in rats and in sea bass. In our current studies, we found that nucleolin, the classic histone chaperon, was able to physically bind to hif-1α mRNA and maintained its stability. Knocking down nucleolin by shRNA obviously reduced HIF-1α protein induction in response to nickel treatment resulting from the impaired expression of hif-1α mRNA. Both the promoter luciferase assay and mRNA degradation rate assay by ActD clearly showed that nucleolin knocking down did not change hif-1α transcription, but rather affected its mRNA stability. Further work found JNK2 could regulate nucleolin expression and subsequently affect its function in stabilizing hif-1α mRNA, by which we provided more evidence for the oncogenic role of JNK2 and nucleolin in regulating the cancer environments by controlling HIF-1α expression. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 2208. doi:1538-7445.AM2012-2208