Abstract 2121: Resveratrol regulates phospho-serine 81 androgen receptor and its stability to inhibit growth of prostate cancer cells

Abstract

Abstract Resveratrol, a polyphenol, has been reported as a cell growth inhibitor of various types of cancer. The molecular mechanisms of resveratrol are involved in different cellular signal transductions such as anti-inflammation and anti-oxidation. Resveratrol inhibits growth of prostate cancer cells through regulating cyclin-dependent kinases, anti-apoptotic proteins, and pro-apoptotic proteins. Notably, resveratrol may down-regulate androgen receptor (AR) and its downstream gene to inhibit prostate cancer cell growth. In previous study, serine 81 phosphorylation of AR increases its protein stability and transcriptional activity, which promotes prostate cancer cell growth. The aim of this study is to investigate whether AR serine 81 phosphorylation would be a target of resveratrol in prostate cancer cells. Our data indicate that resveratrol inhibited the growth of prostate cancer cell line, LNCaP, with the decrease of AR phospho-serine 81 levels. Moreover, AR protein stability was declined by resveratrol without affecting AR mRNA expression. Indeed, resveratrol inhibited AR transcriptional activity in results of AR reporter assay and PSA level in LNCaP cells. In summary, the results suggest that resveratrol might inhibit growth of LNCaP cells, at least, via decreasing phospho-serine 81 of AR, AR protein stability, and AR transcriptional activity. Citation Format: Yu-Ting Peng, Tzu-Yin Chen, Mei-Chih Chen, Eugene Lin, Ho Lin. Resveratrol regulates phospho-serine 81 androgen receptor and its stability to inhibit growth of prostate cancer cells. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 2121. doi:10.1158/1538-7445.AM2014-2121