Abstract 2091: IL-8 mRNA gene expression up-regulated level in both primary culture cell and tissue biopsy from gastric cancer patient without Helicobacter pylori infection and down- regulated level predict clinical response after chemo

Abstract

Abstract Background: There is some recent in vitro study reported that IL-8 mRNA gene had over expression when Helicobacter pylori was co- infected. In the Northern Thai population, we found independently IL-8 mRNA gene expression significantly rising level in gastric tissue biopsies when comparing to AGS cell line, and mainly in non-cag A H. pylori infection or without H. pylori infection. Aim: The author aim to demonstrate the IL-8 mRNA gene expression level in vitro and in vivo study in the same host in order to see the change of its level in primary culture cell in same host gastric cancer patients, tissue biopsy, and AGS cell line. The comparative level before and after chemotherapy treatment were targeted for clinical response prediction. Material and Methods: The control background of corpus normal epithelial and gastric adenocarcinoma biopsy tissues were treated for primary cell culture with single cell isolation technique. The histopathology, background atrophy, and H. pylori infection was confirmed. Biopsy tissues from tumor site also were collected at the same time before and after chemotherapy treatment in patient who received neo-adjuvant chemotherapy by FOLFOX IV regimen due to locally advance stage and unresectable tumor. The IL-8 mRNA gene expression was tested by relative quantitation real time RT-PCR. Results: IL-8 mRNA gene expression can be significantly higher up-regulated level to 1.5-2.2 folds in gastric cancer tissue biopsy comparing to from 0.35-1.16 fold of primary culture cell without H. pylori infection when comparing to normal background of gastric epithelial tissue, and AGS cell line. The IL-8 mRNA gene expression down-regulated level is also remark decreased and/or undetected after chemotherapy treatment in correlation with patients who had well clinical response as a preliminary result. Conclusion: We first demonstrated that the IL-8 mRNA gene expression is detected in primary gastric adenocarcinoma cell and its up-regulated level in tissue biopsies independently related to H. pylori infection in the same gastric cancer host background. The pilot sample size is needed to confirm in further clinical trial on chemotherapy as a new biomarker for advance gastric cancer treatment. Citation Format: Sirikan Limpakan (Yamada), ML.Bandhuphat Chakrabandhu, Shunji Kato, Pattama Wongsirisin. IL-8 mRNA gene expression up-regulated level in both primary culture cell and tissue biopsy from gastric cancer patient without Helicobacter pylori infection and down- regulated level predict clinical response after chemo [abstract]. In: Proceedings of the 106th Annual Meeting of the American Association for Cancer Research; 2015 Apr 18-22; Philadelphia, PA. Philadelphia (PA): AACR; Cancer Res 2015;75(15 Suppl):Abstract nr 2091. doi:10.1158/1538-7445.AM2015-2091