Abstract 208: Reliable Biomimetic Culture System for Pig and Human Heart Slices

Abstract

Rationale: Heart failure is the number one killer and drug induced cardiotoxicity is a major cause of market withdrawal. The lack of availability of culture systems for human heart tissue that is functionally and structurally viable for more than 24 hours is a limiting factor in validation of novel heart failure therapies as well as reliable cardiotoxicity testing. Therefore, there is an urgent need to develop a reliable system for culturing human heart tissue for testing drug efficacy and toxicity. Objective: To develop a reliable method to culture pig and human heart slices under full physiological conditions for a period of time sufficient to test therapeutic efficacy and acute drug toxicity. Methods and Results: Here we describe a novel biomimetic culture system that maintains full viability and functionality of human and pig heart slices (300 μm thickness) for 6 days in culture through optimization of the medium and culture conditions with continuous electrical stimulation at 1.2 Hz and oxygenation of the medium. Functional viability of these slices over 6 days was confirmed by assessing their calcium homeostasis, twitch force generation, and response to β-adrenergic stimulation. Temporal transcriptome analysis using RNAseq at day 2, 6, and 10 in culture confirmed overall maintenance of normal gene expression for up to 6 days, while over 500 transcripts were differentially regulated after 10 days. Electron microscopy demonstrated intact mitochondria and Z-disc ultra-structures after 6 days in culture under our optimized conditions. This biomimetic culture system was successful in keeping human heart slices completely viable and functionally and structurally intact for 6 days in culture. We also used this system to demonstrate the effects of a novel gene therapy approach in human heart slices. Conclusions: We have developed and optimized a reliable and easily reproducible culture system for pig and human heart slices as a platform for testing the efficacy of novel heart failure therapeutics as well as reliable testing of cardiotoxicity in a 3D heart model.