Abstract 2057: Protocadherin 10-mediated suppression of tumorigenesis and metastasis in colorectal cancer

Abstract

Abstract Colorectal cancer (CRC) is one of the leading types of cancer death worldwide, and over one third of these patients will die of their disease mainly due to distant metastasis. Genomic deletion that occurs at tumor suppressor loci is a common genetic aberration in CRC. By using loss of heterozygosity analysis, we identified Protocadherin 10 (PCDH10) as a novel tumor suppressor gene in CRC. Ectopic expression of PCDH10 reduced cancer cell proliferation, anchorage-independent growth, migration and invasion, as well as increased serum starvation-induced apoptosis in vitro. Subcutaneous injection of PCDH10-expressing CRC cells in SCID mice showed the reduction of tumor growth compared with that observed in mock-inoculated mice. Furthermore, via intrasplenic implantation, the re-expression of PCDH10 in silenced cells restrained liver metastasis and improved survival in SCID mice. The molecular mechanisms of PCDH10-mediated tumor suppression are involved in the up-regulation of p53-p21 pathway and down-regulation of active β-catenin. Taken together, PCDH10 could restrain cell growth and epithelial-to-mesenchymal transition, and thus inhibit the development and progression of CRC. Keywords: PCDH10, tumor suppressor gene, colorectal cancer, molecular mechanism Category : MCB04 Oncogenes and Tumor Suppressor Genes Citation Format: yu-lin hung. Protocadherin 10-mediated suppression of tumorigenesis and metastasis in colorectal cancer. [abstract]. In: Proceedings of the 106th Annual Meeting of the American Association for Cancer Research; 2015 Apr 18-22; Philadelphia, PA. Philadelphia (PA): AACR; Cancer Res 2015;75(15 Suppl):Abstract nr 2057. doi:10.1158/1538-7445.AM2015-2057