Abstract 2030: FRK regulates glioma cell progression by promoting N-cadherin/β-catenin complex formation

Abstract

Abstract Fyn-related kinase (FRK), a member of Src-related tyrosine kinases, is recently reported to function as a potent tumor suppressor in several cancer types. Our previous studies has also shown that FRK over-expression inhibited the migration/invasion, proliferation and promoted apoptosis of glioma cells. However, the mechanism of FRK on glioma cell migration/invasion, proliferation and apoptosis is still not clear. In this study, we found that FRK over-expression increased the protein level of N-cadherin, but not that of E-cadherin. Meanwhile, FRK over-expression promoted β-catenin translocation to the plasma membrane, where it formed complex with N-cadherin, while inhibited β-catenin translocation to the nucleus. In addition, down-regulation of N-cadherin by siRNA promoted the migration/ invasion proliferation and inhibited apoptosis of glioma U251 and U87 cells. Interestingly, N-cadherin down-regulation abolished the effect of FRK on glioma cell migration/invasion, proliferation and apoptosis. In summary, these results indicate that FRK regulates glioma cell progression by promoting N-cadherin/β-catenin complex formation. Citation Format: Qiong Shi, Xu Song, Haoping Yan, Jun Wang, Weijian Zhang, Jinxia Hu, Xiuping Zhou, Rutong Yu. FRK regulates glioma cell progression by promoting N-cadherin/β-catenin complex formation. [abstract]. In: Proceedings of the 106th Annual Meeting of the American Association for Cancer Research; 2015 Apr 18-22; Philadelphia, PA. Philadelphia (PA): AACR; Cancer Res 2015;75(15 Suppl):Abstract nr 2030. doi:10.1158/1538-7445.AM2015-2030