Abstract 2020: Determination of parameters to understand the metabolism in the tumor microenvironment by in vivo microdialysis

Abstract

Abstract The tumor microenvironment presents a unique surrounding with more favorable conditions for the cancer cells to growth thrive and interact than for the host cells. To obtain a better understanding of cancer biology and pathophysiology, information about the physiological state of the tumor microenvironment is crucial. By using the in situ microdialysis technique we can quantitate relevant modulators in the interstitial space of the tumor. The current set of experiments demonstrates the benefits of the use of microdialysis in freely-moving awake tumor-bearing animals. Following inoculation, growth was monitored continuously and upon an average tumor volume of 400 mm3, the animals underwent surgical implantation of microdialysis probes. The next day, the sample collection was initiated by perfusion of the implanted probes with dialysate fluid. Samples were continuously collected over several hours and stored at -80C. Levels of analytes indicative of metabolic and physiological conditions of the tumor microdialysis were quantified by EIA and/or LC-MS/MS analysis. The microdialysis technology combined with sensitive analytical methods allows us to quantify several analytes from the tumor microenvironment simultaneously and monitor changes over time. Metabolic activity is an important indicator of tumor growth speed. Depletion of energy can lead to increases in lactate levels and acidification of the tumor microenvironment. Resulting in a reduction of host immunological efficacy. Elucidation of the status of the tumor environment during tumor growth and treatment allows for better translational models. Citation Format: Mariette Suzan Heins, Gunnar Flik. Determination of parameters to understand the metabolism in the tumor microenvironment by in vivo microdialysis [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2024; Part 1 (Regular Abstracts); 2024 Apr 5-10; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2024;84(6_Suppl):Abstract nr 2020.