Abstract 1981: Cell free DNA promotes malignant transformation in non-tumor cells

Abstract

Abstract Cell free DNA (cfDNA) are present at various biological fluids and when released by tumor cells it can contribute with the induction of pro-tumorigenic events, such as malignant transformations and metastasis. In this work we evaluated the effects of tumor derived cfDNA, isolated from blood of prostate cancer patients, in the non-tumoral prostate cellular linages RWPE-1 and PNT-2. We quantified, characterized and evaluated the function of the isolated cfDNA by qPCR of tumor progression related genes and MS-metabolomics. The results showed that the isolated cfDNA was able to induce the increase expression of MMP9 and CD44 in the normal prostate cellular linages, as well as to alter the consumption of tryptophan by the cells, these changes can indicate the sifting of the cells metabolism in favor of a tumoral profile. Those results demonstrate the potential of tumor derived cfDNA to alter receptor cells profiles, triggering malignant transformation events, making them a good target for new therapies and molecular targets for diagnosis. Citation Format: Aline Gomes Souza, Mario Machado Martins, Victor Alexandre Bastos, Natassia Caroline Resende, Paula Sousa Santos, Karina Marangoni, Luiz Ricardo Goulart, Vivian Alonso Goulart. Cell free DNA promotes malignant transformation in non-tumor cells [abstract]. In: Proceedings of the Annual Meeting of the American Association for Cancer Research 2020; 2020 Apr 27-28 and Jun 22-24. Philadelphia (PA): AACR; Cancer Res 2020;80(16 Suppl):Abstract nr 1981.