Abstract
Abstract Background: Non-small-cell lung carcinoma (NSCLC) accounts for 85% of all malignant lung tumors. Our group previously identified Tripartite Motif 14 (TRIM14) as a component of a prognostic multigene expression signature for NSCLC patients. TRIM14 belongs to a conserved family of Tripartite Motif-encoding genes that are involved in a broad range of biological processes, such as transcriptional regulation, cell proliferation, and apoptosis. However, TRIM14 itself is poorly understood, especially in the context of cancer progression. Here we investigate the biological function of TRIM14 in NSCLC. Methods: The expression of TRIM14 was modified in human NSCLC cell lines (NCI-H1395, NCI-H1650 and NCI-H157) by lentivirus-mediated overexpression and short-hairpin RNA-mediated silencing. Effects were assessed by examining in vitro cell proliferation and anchorage-independent growth, and in vivo tumorigenicity in mice. Results: In vitro studies show that TRIM14 overexpression in NSCLC cells suppresses proliferation and anchorage-independent growth, whereas knock-down of TRIM14 expression promotes cell proliferation and colony formation. In vivo studies demonstrate that knock-down of TRIM14 expression significantly increases tumor growth in immunodeficient mice. Conclusion: This is the first study to suggest that TRIM14 may function as a tumor suppressor gene in lung cancer, affecting cell proliferation and anchorage-independent growth in vitro and tumor growth in mice. (Supported by Canadian Cancer Society Research Institute grant #020527) Citation Format: Josephine Hai, Ming-Sound Tsao. Tripartite motif 14 (TRIM14) is a putative tumor suppressor gene in non-small-cell lung cancer. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 1979. doi:10.1158/1538-7445.AM2013-1979
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