Abstract 193: Knockdown of Nucleosome Assembly Protein 1-Like 1 Promotes Dimethyl Sulfoxide-Induced Differentiation of P19CL6 Cells into Cardiomyocytes

Abstract

Various stem cell populations have the potential for cardiac repair and regeneration after transplanted into ischaemic injury heart. However, the low generation of new cardiomyocytes limits the clinic outcomes of stem cell therapy to a great extent. To develop novel strategies for enhancement the efficacy of stem cell differentiation into cardiomyocytes, we employed iTRAQ-coupled 2-D LC-MS/MS proteomics technology workflow to identify novel proteins which could promote P19CL6 cells differentiation into cardiomyocytes induced by dimethyl sulfoxide dimethyl sulfoxide (DMSO). 213 differentially expressed proteins were identified during differentiation of P19CL6 cells into cardiomyocytes. Nine proteins, Nap1L1, Sub1, Hspa5, Ywhaz, Sod1, Prdx2, Mif, Anp32a and Eef2, were confirmed by the real-time polymerase chain reaction (PCR) and western blotting respectively. iTRAQ ratios showed the down-regulation of nucleosome assembly protein 1-like 1 (Nap1L1) during the differentiation of P19CL6 cells into cardiomyocytes, which was confirmed by real-time PCR and western blot results. Further study indicated that knockdown of Nap1L1 expression promoted DMSO-induced cardiomyocytes differentiation in P19CL6 cells characterized by the increased expresison of cardiac specilal transcription factors (GATA4, MEF2C) and proteins (cTNT, ANP and BNP). This work suggested that Nap1L1 play an important role in DMSO-induced cardiomyocytes differentiation of P19CL6 cells. It also provides new insights and platforms for stem cell therapy in cardiovascular disease.