Abstract 1857: Prognostic biomarker for SNP (-617C-A) in ARE-like loci of the NRF2 gene in lung adenocarcinoma

Abstract

Abstract Purpose: We here report that SNP (c.-617C>A; rs6721961) in the ARE-like region of NRF2 gene is associated with Japanese non-smoking female patients with adenocarcinoma and their overall survival. Experimental Design: We prepared genomic DNA samples from 387 Japanese patients with primary lung cancer and detected a SNP (-617C>A) in NRF2 gene by the rapid genetic testing method we developed in this study. We analyzed the association between the SNP in the NRF2 gene and overall survival. Results:Patients harboring wild-type -617C/C, -617C/A, and -617A/A alleles numbered 216 (55.8%), 147 (38.0%), and 24 (6.2%), respectively. Multivariate logistic regression models revealed that SNP homozygote (-617A/A) was significantly related to gender. Its frequency was four-fold higher in female patients than in males (10.8% female vs 2.7% male) and was associated with female non-smokers with adenocarcinoma. Interestingly, lung cancer patients carrying the SNP homozygous alleles (-617A/A) and the 309T (WT) allele in the MDM2 gene exhibited remarkable survival over 1,700 days after surgical operation (log-rank p=0.021). Conclusion: SNP homozygous (-617 A/A) alleles in the NRF2 gene are associated with female non-smokers with adenocarcinoma and regarded as a prognostic biomarker for assessing overall survival of patients with lung adenocarcinoma. Citation Format: Yasuko Okano, Yasushi Ichikawa, Yohei Miyagi, Keiichi Kondo, Yutaka Natsumeda. Prognostic biomarker for SNP (-617C-A) in ARE-like loci of the NRF2 gene in lung adenocarcinoma. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 1857. doi:10.1158/1538-7445.AM2014-1857