Abstract 1854: P16 immunoreactivity in endocervical tubo-endometrial metaplasia (TEM) and correlation with high risk HPV (HRHPV) status by chromogenic in-situ hybridization (HPV-CISH) and hybrid capture 2 assay

Abstract

Abstract Background Several studies have shown that endocervical TEM can intensively over express p16 and involve more than 50% of the cells. It could be speculated that TEM may be a precursor lesion of cervical glandular intraepithelial neoplasm. The aim of this study is to evaluate endocervical TEM p16 overexpression pattern and further investigate its potential driven mechanisms by correlating with HRHPV status. Method Our search included cervical biopsy, LEEP, conization with TEM diagnosis from January 2005-May 2013. Only cases with concurrent Pap smear with HRHPV testing were selected. HRHPV detected in liquid based cytology is by Hybrid Capture 2 Assay. Surgical specimens containing concurrent squamous dysplasia were excluded due to potential confounding by HRHPV positivity in dysplastic squamous epithelium. P16 stain was performed and graded as negative, focal+ (<50% cytoplasmic and nuclear stain) and diffuse+ (>50% cytoplasmic and nuclear stain). HRHPV data was collected from Pap reports. HPV-CISH assays will be performed for all cased with positive HRHPV detected in liquid based cytology. Result: Our search generated 159 cases with TEM. Only 81 cases had concurrent Pap smear with HRHPV testing. 48 cases were excluded due to squamous dysplasia. A total of 33 patients with an age range of 20-59 were available for the study. Twenty nine out of 33 had negative HRHPV in liquid based cytology; in which, p16 stain results as: 8/29 negative, 14/29 focally +. 7/29 diffusely +. Four out of 33 had positive HRHPV detected in liquid based cytology; in which, p16 stain results as: 1/4 negative, 3/4 diffusely +. HPV-CISH assays were performed in all 4 cases and they were negative. Conclusions P16 overexpression in TEM can be intense and involve more than 50% of the cells but the pattern is heterogenous and positivity never involves 100% of the cells. Neither HPV-CISH nor Hybrid Capture 2 Assay detects HRHPV in TEM. P16 overexpression in TEM is most likely HRHPV-independent mechanisms. . Note: This abstract was not presented at the meeting. Citation Format: Shobhana Talukdar, Gomez-Gelvez Juan, Thomas E Buekers, Ziying Zhang. P16 immunoreactivity in endocervical tubo-endometrial metaplasia (TEM) and correlation with high risk HPV (HRHPV) status by chromogenic in-situ hybridization (HPV-CISH) and hybrid capture 2 assay. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 1854. doi:10.1158/1538-7445.AM2014-1854