Abstract 1839: Long non-coding RNA SS-001 regulates proliferation, cell cycle, and migration in esophageal adenocarcinoma cells.

Abstract

Abstract Esophageal adenocarcinoma (EAC) is one of the most rapidly increasing cancers in Western countries, but its underlying molecular mechanisms have not yet been completely elucidated. Recently, the discovery of long non-coding RNAs (lncRNAs) has added a new layer of complexity to the molecular architecture, identifying these molecules as emerging key regulators of diverse biological pathways. Increasing evidence shows that lncRNA dysregulation can lead to diseases such as cancer, but their potential involvement in EAC is not yet well-understood. In this study, we identified a set of lncRNAs that were differentially expressed in esophageal cancers vs. normal tissues by massively parallel RNA sequencing. Expression levels were validated using quantitative real-time PCR. One lncRNA, designated SS-001, was significantly overexpressed and hypomethylated in EAC cell lines and tissues vs. normal esophageal epithelial cells and adjacent non-tumor tissues, respectively. SS-001 is an intergenic lncRNA located on chromosome 21. In vitro assays in EAC cell lines demonstrated that knockdown of SS-001 resulted in inhibition of cell proliferation, the cell cycle, and migration. Moreover, patients with high SS-001 expression levels had a relatively poor prognosis. Our findings suggest that SS-001 may play a significant role in the development or progression of EAC and has the potential to serve as a prognostic biomarker. Citation Format: Jee Hoon Song, Stephen J. Meltzer. Long non-coding RNA SS-001 regulates proliferation, cell cycle, and migration in esophageal adenocarcinoma cells. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 1839. doi:10.1158/1538-7445.AM2013-1839