Abstract 1732: The analysis of stem cell property and mesenchymal feature of anaplastic pancreatic cancer

Abstract

Abstract Background: Anaplastic pancreatic cancer (APC) is the subtype of pancreatic ductal adenocarcinoma (PDAC). APC is poorer prognosis than ordinary PDAC. However, it is unclear what feature of APC is different from that of ordinary PDAC. Our previous studies suggested that the angiogenesis and the tolerance of hypoxia might related to the higher in vivo proliferation of APC than ordinary PDAC. Purpose: We tried to estimate the difference between APC and PDAC using SP cells which reflected cancer stem-like cell and drug resistance and western blotting of E-cadherin and vimentin which reflected epithelial-mesenchymal transition. Materials and Methods: For SP cell analysis, OCUP-A1 and OCUP-A2 were used as APC cell lines and Panc-1 and MIAPaCa2 were used as ordinary PDAC cell lines. These cells were incubated in the medium including Hoechst 33342 with or without verapamil. Then, propidium iodide was added. And the analysis was performed using FACS Aria II. For western blotting, OCUP-A1 and OCUP-A2 were used as APC cell lines. And breast cancer cell line MCF-7 was used as control. Results: The proportion of SP cells in all cells was as follows; OCUP-A1/ OCUP-A2/ Panc-1/ MIAPaCa2 = 1.8±0.28%/ 1.7±0.12%/ 1.1±0.20%/ 0.6±0.058%. The proportion of SP cells of OCUP-A1 and OCUP-A2 were similar to that of Panc-1. However the proportion of SP cells of OCUP-A1 and OCUP-A2 significantly larger than that of MIAPaCa2. In western blotting, the expression of vimentin was observed and the expression of E-cadherin was lost in OCUP-A1 and OCUP-A2. Inversely, the expression of E-cadherin was observed and the expression of E-cadherin was lost in MCF-7. Discussion: It was reported that SP cells might contribute to tumorigenesis and drug resistance the same as cancer stem-like cell. Our current study suggested that larger proportion of SP cells in APC might induce higher malignancy of APC. Also, it was reported that mesenchymal feature might related to cancer stem cell properties. In this study, it was suggested that APC cell lines might have mesenchymal feature. However, it has been unclear whether mesenchymal feature of APC would affect the proportion of SP cells of APC from this study. So, we need to confirm the correlation with cancer stem sell property and mesenchymal feature of APC. Conclusion: The large proportion of SP cells and the mesenchymal feature might contribute to higher malignant property of APC. Citation Format: Kenjiro Kimura, Kotaro Miura, Ryosuke Amano, Sadaaki Yamazoe, Naoki Kametani, Kohei Nishio, Masaichi Ohira, Kosei Hirakawa. The analysis of stem cell property and mesenchymal feature of anaplastic pancreatic cancer. [abstract]. In: Proceedings of the 107th Annual Meeting of the American Association for Cancer Research; 2016 Apr 16-20; New Orleans, LA. Philadelphia (PA): AACR; Cancer Res 2016;76(14 Suppl):Abstract nr 1732.