Abstract 163: Identification and characterization of TGFBI in circulating tumor cell subline from pancreatic cancer cell line

Abstract

Abstract Distant metastasis to liver, lung, brain, or bone occurs via circulating tumor cells (CTCs). We hypothesized that a subset of CTCs had more malignant features than tumor cells at the primary site. We established a highly malignant cell line, Panc-1-CTC, derived from the human pancreatic cancer cell line Panc-1 (Panc-1-P) using an in vivo selection method. Panc-1-CTC cells exhibited more profound migratory and invasive abilities than Panc-1-P cells in vitro. In addition, Panc-1-CTC cells had a higher tumor-forming ability than Panc-1-P cells in vivo. To investigate whether a difference in malignant phenotypes exists between Panc-1-P and -CTC cells, we performed comprehensive gene expression array analysis. As a result, Panc-1-CTC significantly expressed transforming growth factor beta-induced (TGFBI), an extracellular matrix protein, more abundantly than did Panc-1-P cells. TGFBI is considered to regulate cell adhesion, but its functions remain unclear. In the present study, knockdown of TGFBI reduced cell migration and invasion abilities, whereas overexpression of TGFBI increased both abilities. Moreover, elevated expression of TGFBI was associated with poor prognosis in patients with pancreatic cancer. Taken together, TGFBI might be targeted for cancer therapy and its expression could be a biomarker for the prediction of prognosis in pancreatic cancer. Citation Format: Tomoki Muramatsu, Taku Sato, Minoru Tanabe, Johji Inazawa. Identification and characterization of TGFBI in circulating tumor cell subline from pancreatic cancer cell line [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2019; 2019 Mar 29-Apr 3; Atlanta, GA. Philadelphia (PA): AACR; Cancer Res 2019;79(13 Suppl):Abstract nr 163.