Abstract 1571: Tumor cell panels: New tools in genomic era.

Abstract

Abstract Increased understanding of cancer genome is affecting every corner of cancer research. Although human tumor cell lines have been used as essential tools for decades, there are only a few cell line panels have been developed for drug screening. The most widely used NCI60 human tumor cell line panel was composed by selecting cell lines to represent nine subtypes of cancer. The rising of targeted therapeutics has led to new generation anticancer agents. However, it's difficult to predict clinical response to these agents because of the genetic diversity and genomic heterogeneity in human cancers. Therefore, there is a gap between the new knowledge of cancer genome and the cell line based platforms for both basic and translational research. Here, we show that new generation tumor cell panels are filling the gap. The panels were generated by selecting authenticated cell lines derived from variant cancer types, and annotated with genetic alteration information generated by large scale sequencing projects such as the Catalog of Somatic Mutations in Cancer (COSMIC) and the Cancer Cell Line Encyclopedia (CCLE). There are more than 25 tumor cell line panels, which include hundreds of cell lines, have been developed in this initiative. They cover 18 most common cancer types, such as lung, breast, colon, pancreatic cancer and so on. To capture the genetic diversity of cancer, each panel includes cell lines with varying gene mutation complexity. To further facilitate targeted drug discovery, the molecular signature tumor cell line panels focus on individual driver genes, critical protein kinases, transcription factors and cell signaling pathways. Those panels have been analyzed to verify gene mutation, gene expression, protein expression and bio-functions. Therefore, the new generation tumor cell panels provide powerful tools to accelerate the discoveries in basic cancer research, compound screening, biomarker selection, pathway analysis, and targeted therapeutic development. Citation Format: Chris White, Lysa-Anne Volpe, Luping Chen, Anupreet Bal, Michael Jackson, John Foulke, Fang Tian. Tumor cell panels: New tools in genomic era. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 1571. doi:10.1158/1538-7445.AM2013-1571