Abstract 154: [6]-Shogaol inhibits cell growth and induces apoptosis in non-small-cell lung cancer (NSCLC) cells by regulating multiple targets

Abstract

Abstract Non-small-cell lung cancer (NSCLC) is the leading cause of cancer mortality worldwide. Despite substantial progress in developing chemotherapeutics for the treatment of NSCLC, primary and secondary resistance limits the success of these therapies. NSCLC cells exhibit different EGFR mutations and EGFR has extensive cross-talk with other cell signaling pathways. Therefore, suppression of the inappropriate expression of multiple targets is considered to be a rational therapeutic and preventive strategy for the management of NSCLC. Our initial molecular-target oriented virtual screening revealed that the ingredients of ginger, including [6]-gingerol, [6]-shogaol and [6]-paradol appeared to be potential candidates for the prevention and therapy of NSCLC cells. Among the three compounds, [6]-shogaol showed the greatest inhibitory effect on growth and soft agar colony formation of NSCLC cells. [6]-Shogaol induced cell cycle arrest (G1 or G2/M) and apoptosis. Furthermore, [6]-shogaol interacted with PI3-K and AKT and inhibited AKT kinase activity. Finally, [6]-shogaol increased the expression of p27, which is one of the target proteins in the AKT signaling pathway. The present results clearly indicate that [6]-shogaol exerted a strong anti-cancer effect and can be regarded as useful tool for the treatment of NSCLC. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 154. doi:1538-7445.AM2012-154