Abstract 15017: Chromatin Regulators as Potential Players in Atrial Fibrillation: Locus Analysis and Regulatory Role Investigation

Abstract

Background: Atrial fibrillation (AF), a prevalent cardiac arrhythmia, has multiple biomarkers identified, yet their roles remain partially understood. Research Questions and Aims: The study sought to investigate the expression and prognostic value of Chromatin Regulators (CRs) in AF, their correlation with the immune microenvironment, and to identify potential prognostic markers, therapeutic drugs, and miRNAs. Methods: We filtered three GEO database datasets containing AF and normal samples, identifying 23 differentially expressed CRs. Sequence analysis and protein-protein interaction (PPI) network construction led to the identification of 10 AF prognosis-related CRs. The biological roles and potential pathways of these CRs were analyzed through Gene Ontology (GO) and ssGSEA algorithm. Moreover, we examined the correlation between hub genes and immune cells/immune function, identifying the top 5 immunity-relevant CRs. Results: Ten CRs linked to AF prognosis were identified, achieving an AUC of 0.901997 in a nomogram model. GO analysis demonstrated the involvement of these CRs in processes associated with cardiovascular diseases. The ssGSEA algorithm showed a connection between the identified CRs and immune infiltration. The study also identified the top 5 immunity-related CRs, and potential drugs and miRNAs for AF therapy. Conclusions: This study elucidates the role of specific CRs in AF, presenting a promising risk model and potential therapeutic targets. Further validation and evaluation of potential therapeutic targets' efficacy and safety are required. This underscores CRs as potential biomarkers and therapeutic targets for AF.