Abstract 1455: Vitamin D receptor (VDR) gene polymorphisms among Sudanese females with breast cancer attending Radio and Isotope Center of Khartoum

Abstract

Abstract Background: Vitamin D Receptor (VDR) gene polymorphisms has been reported to be associated with different cancers, including breast cancer. geographical and environmental factors have an impact on vitamin D (VD) metabolism. This study aims to evaluate VDR-gene polymorphisms (Fok1, Bsm1, Taq1, Apa1) and its association with breast cancer risk among Sudanese females. Materials and Methods: this is a case control study done in the Radio and Isotope Center in Khartoum, Sudan. Hundred thirty seven patients and 69 healthy controls were enrolled after informed consent. Blood genomic DNA was extracted using quinidine chloroform method, and single nucleotide polymorphisms (SNPs) Fok1 (T/C) [rs2228570], Bsm1 (G/A) [rs1544410], Taq1 (C/T) [rs731236] and Apa1 (C/A) [(rs7975232] in VDR-gene were detected by PCR and restriction fragment length polymorphism (PCR-RFLP) analysis. Results: VDR-SNPs rs731236 correlated significantly with low risk of breast cancer (OR=0.5, p= 0.002). There was no significant association observed with rs1544410 and rs7975232. Although our study did not show an overall statistically significant association of VDR-SNPs rs2228570 with the risk of breast cancer, we found a 1.3 fold increased risk of breast cancer (OR= 1.3) among females with FF genotype when compared with ff genotype, suggesting that females homozygous for F allele, might be at more risk than females carrying homozygous f allele. Conclusions: These results suggested that, VDR-SNPs rs2228570 may be related to higher risk of breast cancer and VDR-SNPs rs731236 associated with lower risk of breast cancer in Sudanese females. Key words: Breast Cancer, VDR, Polymorphisms, SNPs Citation Format: Sara O. Yousif, Amar M. Ismail, Abdelrahim O. Mohamed, Abdelrahim O. Mohamed. Vitamin D receptor (VDR) gene polymorphisms among Sudanese females with breast cancer attending Radio and Isotope Center of Khartoum [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2017; 2017 Apr 1-5; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2017;77(13 Suppl):Abstract nr 1455. doi:10.1158/1538-7445.AM2017-1455