Abstract 13658: Cholesterol Content of Lipoprotein Particles and Progression of Coronary Artery Calcification From Different Methods in ELSA-Brasil Study - A Metabolomic Approach

Abstract

Background: Cholesterol content (CC) of lipoprotein particles is heterogeneous according to size and type. Research Question: The association of CC with carotid artery calcium (CAC) progression in different subclasses is not established. Aim: To analyze the association between CC of lipoprotein particles and CAC progression in ELSA-Brasil cohort study. Methods: Data from 883 participants (50.5±7.3 years, 50.7% women) without cardiovascular disease and statin use were analyzed. The CC in mmol/l was assessed by high-throughput proton Nuclear Magnetic Resonance metabolomics platform in 14 subclasses according to type and size at baseline. A 5-year CAC progression was assessed by computed tomography and calculated by log transformed, square root transformed and by methods of Hokanson and of Berry. The association of CC with CAC progression was analyzed by generalized linear models adjusted by sociodemographic (age, sex, race, education, health insurance), lifestyle (physical activity, alcohol consumption, smoking), comorbidities (hypertension, diabetes, obesity and total cholesterol ≥200mg/dL) and further adjusted by particle concentration. Results: A total of 15.6% of sample (n=138) presented a positive follow-up CAC score. The CC of medium VLDL, IDL, and LDL (large, medium and small) was associated with CAC progression by Berry method - Table 1. However, only the CC in medium VLDL (β=3.059 [95%CI: 0.040; 6.078], p=0.047) and large LDL (β=1.007 [95%CI: 0.342; 1.673], p=0.003) remained associated after adjustment for particle concentration. The CC of small HDL was associated with CAC progression by square root method (β=13.427 [95%CI: 0.487; 26.367], p=0.042), but lost significance after adjustment for particle concentration. Conclusions: The CC of medium VLDL and large LDL was associated with CAC progression by Berry method, beyond sociodemographic, lifestyle, comorbidities and particle concentration in a subsample of ELSA-Brasil participants.