Abstract 1348: Association between genetic variation at the AKT1 and PTEN genes, and in the 8q24 locus, and the risk of testicular germ cell tumor.

Abstract

Abstract Background There is strong evidence that genetic variation influences the risk of testicular germ cell tumor (TGCT). The oncogene AKT1, tumor suppressor gene PTEN and the 8q24 locus play important roles in cancer development, and investigation of their role in TGCT is warranted. Methods We investigated the association between risk of TGCT and genetic variation in the AKT1 and PTEN genes, and the 8q24 locus in a Norwegian-Swedish case-parent study. We included 831 triads, 474 dyads and 712 singletons, and 26 single nucleotide polymorphisms (SNPs) were genotyped. First, triads and dyads were included in a likelihood-based association test. To increase the statistical power, case singletons and 3922 unrelated controls from the Swedish TwinGene project were included in a single test for association. Interaction terms were included in the statistical model to examine if the allelic effect on TGCT risk was modified by histological subgroup, country of origin or parent of origin. Odds ratios (OR) and 95% confidence intervals (95% CI) were calculated and Bonferroni correction (Pbonf) was used to adjust for multiple testing. Results In the case-parent triad analyses, none of the 26 SNPs were significantly associated with TGCT. Of the 23 SNPs investigated in the combined study, one SNP in the PTEN gene, the rs11202586, remained associated with TGCT risk after adjusting for multiple testing (OR=1.16, 95% CI=1.06-1.28, Pbonf=0.040). We found no difference in risk according to histological subgroup, parent of origin or between countries. Conclusions Our findings suggest that genetic variation in the PTEN gene influences the risk of TGCT. Table. Odds ratios (ORs) for associations between polymorphisms in AKT1, PTEN and 8q24 locus and TGCT risk in the combined case-parent/case-control study. Gene Gene name SNP Allele1* Allele2 Genomic position CHR OR 95% CI P Pbonf# AKT1 v-akt murine thymoma viral oncogene homolog 1 rs2494731 C G 104308725 14 0.93 0.86–1.01 0.071 1.000 rs2494739 C T 104318034 0.96 0.83–1.11 0.594 1.000 rs2494752 A G 104334653 0.95 0.81–1.11 0.502 1.000 rs4983387 A G 104339273 1.07 0.95–1.22 0.283 1.000 PTEN phosphatase and tensin homolog rs1234212 C T 89598872 10 0.99 0.92–1.07 0.824 1.000 rs11202586 C T 89602004 1.16 1.06–1.28 0.002 0.040 rs1234221 A C 89606459 0.97 0.89–1.06 0.537 1.000 rs1234220 A G 89635453 1.16 1.03–1.31 0.018 0.414 rs1234219 A G 89639557 1.09 0.95–1.25 0.221 1.000 rs2299939 A C 89647130 1.04 0.95–1.15 0.386 1.000 rs12357281 C G 89690651 1.05 0.91–1.20 0.534 1.000 rs2248293 C T 89697245 0.95 0.88–1.03 0.233 1.000 rs926091 C T 89711392 0.91 0.82–1.02 0.105 1.000 8q24 locus rs7008482 G T 126336812 8 0.91 0.84–0.99 0.029 0.674 rs13254738 A C 128173525 1.02 0.94–1.10 0.707 1.000 rs6983561 A C 128176062 0.98 0.80–1.19 0.815 1.000 rs16901979 A C 128194098 0.94 0.78–1.15 0.566 1.000 rs6983267 G T 128482487 0.98 0.91–1.05 0.548 1.000 rs7000448 C T 128510352 0.97 0.90–1.05 0.424 1.000 rs1447295 A C 128554220 0.99 0.88–1.11 0.864 1.000 rs4242382 A G 128586755 1.00 0.89–1.13 0.986 1.000 rs7017300 A C 128594450 0.94 0.85–1.05 0.266 1.000 rs7837688 G T 128608542 1.06 0.94–1.19 0.333 1.000 * Reference allele # Bonferroni correction (P multiplied by the number of total SNPs analyses, n = 23). Citation Format: Kristine E. Andreassen, Wenche Kristiansen, Robert Karlsson, Elin L. Aschim, Olav Dahl, Sophie D. Fosså, Hans-Olov Adami, Fredrik Wiklund, Trine B. Haugen, Tom Grotmol. Association between genetic variation at the AKT1 and PTEN genes, and in the 8q24 locus, and the risk of testicular germ cell tumor. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 1348. doi:10.1158/1538-7445.AM2013-1348