Abstract 1345: Automated capture and analysis of circulating tumor cells across different types of tumors in pediatric cancer patients

Abstract

Abstract The clinical utility of blood analysis such as circulating tumor cells (CTCs) has garnered increasing interest in recent years. However very few studies were conducted in pediatric cancer patients especially in brain tumor, osteosarcoma, and leukemia patients due to the lack of an effective tool to isolate/capture CTC from these patients. For example, only three independent studies were published to investigate CTC capture from adult brain tumor patients but zero studies were conducted with pediatric brain cancer patients. Our previous studies showed the high specificity of using cell surface vimentin (CSV) to identify and capture CTCs from various types of adult tumor patients using a manual isolation method. Here we present the first automated system for capturing CTCs across different types of pediatric tumors. This automated system utilizes the Abnova CytoQuest system loaded with the CSV antibody 84-1-coated microfluidic cassette for capturing CTCs from peripheral blood samples. This automated assay was very sensitive because as few as one tumor cell can be captured out from one million normal cells. Based on our preliminary study, our success rate for non-DIPG brain tumor, ALL, and AML patients is 100%. This success provides a potential opportunity for analyzing brain tumor genetic properties using blood samples instead of the invasive tumor biopsy; likewise, this high sensitivity assay opens up clinical opportunities to use this assay for transplant and treatment decision making. Note: This abstract was not presented at the meeting. Citation Format: Izhar S. Batth, Wafik Zaky, Soumen Khatua, Najat C. Daw, Kris M. Mahadeo, Sajad Khazal, Aisha Albert, Wilber Huang, Diva Chen, Jonathan B. Gill, Eugenie Kleinerman, Richard Gorlick, Shulin Li. Automated capture and analysis of circulating tumor cells across different types of tumors in pediatric cancer patients [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2019; 2019 Mar 29-Apr 3; Atlanta, GA. Philadelphia (PA): AACR; Cancer Res 2019;79(13 Suppl):Abstract nr 1345.