Abstract
Abstract Current cancer research focuses on blockading metastasis with the hope of increasing patient survival and prognoses. This research has spurred an interest in metastasis suppressor genes, such as KISS-1, whose role in breast cancer remains controversial. This study focuses on the interactions among one of the proteolytic processing products of the Kiss1 protein (kisspeptin10, KP10), its receptor, KISS1R (GPR54, AXOR12), osteoblasts (bone forming cells), and bone-metastatic breast cancer cells, MDA-MB-231. Previous research suggests that treatment with KP10 reduces the ability of the cancer cells to invade a reconstituted basement membrane (Matrigel) and to migrate in response to osteosarcoma cells. The aim of this present study was to determine if KP10 affected the interaction of MC3T3-E1 osteoblasts and bone-metastatic breast cancer cells. Our laboratory had previously determined that osteoblasts exhibit an inflammatory response when treated with conditioned medium from breast cancer cells. This inflammatory response may contribute to cancer cell proliferation in the bone. This response has not yet been characterized in the presence of KP10. I hypothesized that KP10 would down regulate the inflammatory response of osteoblasts to breast cancer cells. MDA-MB-231 breast cancer cells express theKISS1R; therefore, these cells were treated with KP10 (10-100 nM, 2hr), the medium was collected, and the osteoblasts were treated with it. KP10 significantly decreased the inflammatory response of osteoblasts to breast cancer conditioned media indicated by decreased levels of IL-6 as determined by ELISA. This research has important implications because it provides insight into how KP10 may be acting on either the breast cancer cells or the bone cells. In my future research, I will continue to characterize how KP10 can be used to prevent proliferation or activation of breast cancer metastases in the bone. This work was supported by an undergraduate research grant from the Eberly College of Science of Penn State University. Citation Format: Kaitlyn E. Leahey, Danny R. Welch, Yu-Chi Chen, Karen M. Bussard, Andrea M. Mastro. Deadly KISS, Kisspeptin10 interaction with osteoblasts and breast cancer metastastic cells. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 130. doi:10.1158/1538-7445.AM2014-130
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