Abstract 13.55 A COMPARISON OF THE REGENERATIVE GENE EXPRESSION IN ASCS DERIVED FROM UAL, SAL AND ABDOMINOPLASTY SAMPLES

Abstract

BACKGROUND: Due to their regenerative potential, Mesenchymal stem cells are already in clinical use in several medical specialties. In plastic surgery, mainly the adipose-derived stromal cells (ASCs) are in the focus of recent research to remedy defects across the whole body and to reverse ischemic tissue states. The ability of the regenerative cytokine release is the key mechanism in the therapeutic use of ASCs. Through the intensive research effort of the last decades in the area of liposuction for aesthetic or reconstructive use, various methods and devices have been developed. However which method derives the most potent cells for regenerative applications is still disputed. Here we assess ASCs from Ultrasound assisted (UAL) and suction assisted (SAL) lipoaspirat as well as ASCs from excised fat for their ability to release key cytokines for tissue regeneration. MATERIAL AND METHODS: UAL and SAL lipoaspirate was collected from 5 patients and compared with excisional fat from abdominoplasty samples from the same patients. ASCs were characterized by their surface marker profile CD45- / CD31- / CD34 + by means of FACS and the expression of differentiation cytokines (HGF, FGF-2, MCP-1, SDF-1, VEGF) was compared. RESULTS: There was no significant difference between the release of HGF, FGF-2, SDF-1 and VEGF between the individual samples. However, we observed an increased expression of MCP-1 in the UAL sample. CONCLUSION: Utilizing an elegant study design with the patients serving as their own controls we were able to show that UAL and SAL both derive ASCs comparable to cells from excised fat. This demonstrates these methods have potential to harvest ASCs for autologous fat transfer and regenerative medicine.